Evaluation of Antibacterial Activity of Salvia Officinalis L. Sage Oil on Veterinary Clinical Isolates of Bacteria

In the study except a strain of Dermatophilus congolensis isolated from a dog blood suffering from pyrexia, all bacterial isolates from diseased animals were resistant to discs containing 2 microL sage oil. The only sensitive strain of D. congolensis also showed narrow zone (8 mm) of growth inhibition while the sensitive reference strain of E. coli-382 had 10-12 mm zone of growth inhibition around SEO discs. The MIC for the reference E. coli strain was 0.64 microL/ ml while for D. congolensis strain it was 1.28 l/ ml with all three methods (agar dilution, broth dilution and agar well methods) of MIC determination. Sage oil MIC for four Streptococcus species strains was 2.56 l to 5.12 microL/ ml while for three strains of Pasteurella canis and four strains of Plesiomonas shigelloides was 5.12microL / ml. For screening purpose, disc diffusion assay for antimicrobial activity appeared useful tool. The study revealed that MIC of sage oil could be determined using any of the three (broth dilution, agar dilution or agar well) methods without any significant variation among results.